p-ISSN: 1300-0551
e-ISSN: 2587-1498

Ahmet Melih Şahin1, Osman Fuat Sönmez1, Murat Mengi1, Mehmet Altan1, Muhammet Sait Toprak2, Hakan Ekmekçi2, Şensu Dinçer3, Gökhan Metin1, Lütfi Çakar4

1Department of Physiology, Cerrahpaşa Faculty of Medicine, İstanbul University, İstanbul, Turkey
2Department of Biochemistry, Cerrahpaşa Faculty of Medicine, İstanbul University, İstanbul, Turkey
3Department of Sports Medicine, İstanbul Faculty of Medicine, İstanbul University, İstanbul, Turkey
4Department of Physiology, Faculty of Medicine, Sanko University, Gaziantep, Turkey

Keywords: Diabetes Mellitus, acute exhaustive exercise, oxidative stress, antioxidants


Objective: The aim of this study was to investigate the changes in certain oxidative stress and antioxidant system parameters in response to acute exhaustive exercise (AEE) applied to diabetes-induced rats.

Methods: 16 male Wistar rats were divided diabetes (n=8) and control (n=8) groups. For diabetes induction, 65 mg/kg of streptozotocin (STZ) administered to rats intra-peritoneally. On the fourth day of STZ administiration, blood glucose level was measured and level of above 180 mg/dL was considered as diabetes mellitus (DM). AEE procedure was applied to rats within the same day that they were considered as diabetes. After the exercise protocol, blood samples were collected for biochemical analyses and serum samples were obtained by centrifugation. 3-nitrotyrosine, LPx, protein carbonyl, SOD and GSH, GSH-Px levels were analyzed by using Enzyme-linked immunosorbent assay.

Results: After the exercise, both groups had decreased blood glucose levels compared to pre-exercise levels. There was a significant increase in lactate levels of both groups, compared to pre-exercise levels. In terms of distance run, control group ran approximately two times more distance than the diabetes group. When we compared oxidative stress parameters; Lipid peroxide, 3-nitrotyrosine, and protein carbonyl levels were significantly higher in the diabetes group. Antioxidant parameters; glutathione and glutathione peroxidase were significantly higher in the diabetes group. As for superoxide dismutase levels, it was slightly decreased in the diabetes group, but did not reach statistical significance.

Conclusions: AEE triggers both oxidant and antioxidant systems in diabetic rats.

Cite this article as: Sahin AM, Sonmez OF, Mengi M, Altan M, Toprak MS, Ekmekci H, et al.Effects of acute exhaustive exercise on oxidant and antioxidant system parameters in rats with streptozotocin induced diabetes mellitus. Turk J Sports Med. 2020;55(2):131-37.