The Effect of PRGF on Healing of Clamp-Induced Tendinopathy in Animals
Uğur Diliçıkık1
, Haydar Ali Demirel2, Gürhan Dönmez1, Mustafa Fevzi Sargon3, Emir Birant4, Mahmut Nedim Doral5
1Department of Sports Medicine, Faculty of Medicine, Hacettepe University, Ankara, Turkey
2Faculty of Sport Sciences, Hacettepe University, Ankara, Turkey
3Department of Anatomy, Faculty of Medicine, Hacettepe University, Ankara, Turkey
4Department of Mechanical Engineering, Middle East Technical University Ankara, Turkey
5Department of Orthopedics and Traumatology, Faculty of Medicine, Hacettepe University, Ankara, Turkey
Keywords: PRGF, PRP, tenocyte, collagen
Abstract
Objectives: To investigate the effect of PRGF on the healing of the tendon damage in the Achilles tendinopathy model.
Material and Methods: A preliminary study was designed to obtain more apparent tendinopathy changes in the subculture. Histopathologically, second level compression with a clamp for 1 minute followed by releasing for 3 minutes applying the same mechanical stimulus procedure for four times, was defined as the optimal compression mode for the most optimal damage in terms of tendinopathy. Thirty-four female Sprague Downey type rats were divided into five separate groups and clamped tendon damage was formed on unilateral (left) Acchilles tendons. The first group was a control group (Kontrol: CN); in the second group tissue specimens were taken from the rats immediately after being clamped (K-0); from the group 3, tissue samples were taken 1 week after clamping (K-7). Immediately after the tendinopathy model was applied to the animals in the fourth group (PRGF), the PRGF injection was performed to the subjects from the blood that was obtained from the control group, while an isotonic saline solution was injected into the subjects in the fifth group (SF), and tissue samples from both groups were obtained 1 week after.
Results: The samples were examined by means of a transmission electron microscope. In the K-0 group among the collagenous fibers, vacuoles are observed in the cytoplasm of the edematous areas and fibrocytes, and in comparison, with K-7 and SF group there was a significant increase in fibroblast and collagen fiber synthesis in the PRGF-treated group. Furthermore, fibroblast and new collagen synthesis were observed to be increased in K-7 and SF groups. An intense increase in organelle levels was observed in PRGF group fibroblasts.
Conclusion: This data suggests that PRGF may provide a better healing response in tendons with limited healing and circulation, which enhances the activation in the cells at the beginning of the healing process.
Cite this article as: Dilicikik U, Demirel HA, Donmez G, et al. The effect of PRGF on healing of clamp-induced tendinopathy in animals Turk J Sports Med. 2018;53:51-58
